1. Signaling Pathways
  2. Cell Cycle/DNA Damage
  3. DNA Stain

DNA Stain

There are several different stains that can be used to visualize and photograph DNA after separation by gel electrophoresis. Molecules of the dye adhere to DNA strands and fluoresce under UV light, showing where the bands are within the gel.

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra.

DNA Stain Related Products (87):

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1070
    DBCO-PEG4-TAMRA
    DBCO-PEG4-TAMRA is a PEG-based TAMRA dye and contains a DBCO group, which enables Click Chemistry. The TAMRA dye is a dye widely used in oligonucleotide labeling and automated DNA sequencing applications. DBCO-PEG4-TAMRA is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
    DBCO-PEG4-TAMRA
  • HY-D0049
    6-TAMRA-SE
    6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing.
    6-TAMRA-SE
  • HY-D0942
    Acridine Orange zinc chloride salt
    Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).
    Acridine Orange zinc chloride salt
  • HY-D1543
    Pyronin B
    Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor.
    Pyronin B
  • HY-DY1047
    DFHBI-1T (solution)
    DFHBI-1T (solution) is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.
    Solvent and concentration: DMSO: 20 mM
    DFHBI-1T (solution)
  • HY-DY1067
    Cy5 maleimide (solution)
    Cy5 maleimide (solution) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Solvent and concentration: DMSO: 10 mM
    Cy5 maleimide (solution)
  • HY-D1396
    Br-DAPI
    98.0%
    Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. Storage: Keep away from light.
    Br-DAPI
  • HY-D0115
    7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester
    7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is a blue fluorescent dye for labeling proteins and nucleic acids.
    7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester
  • HY-136247A
    Cyanine 5 Tyramide methyl indole
    Cyanine 5 Tyramide (Tyramide-Cy5) methyl indole is a red fluorescent dye. Cyanine 5 Tyramide is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids. Storage: protect from light.
    Cyanine 5 Tyramide methyl indole
  • HY-125786
    Solvent violet 9
    99.09%
    Solvent violet 9 is a dye for recognizing DNA.
    Solvent violet 9
  • HY-D1086
    6-Carboxy-X-rhodamine, succinimidyl ester
    6-Carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE) is a fluorescent dye for oligonucleotide labeling and automated DNA sequencing.
    6-Carboxy-X-rhodamine, succinimidyl ester
  • HY-W582504
    Chlorotris(triphenylphosphine)copper
    Ligand 98.0%
    Chlorotris(triphenylphosphine)copper (CuCl(TPP)₃) is a DNA-targeted metal complex. Chlorotris(triphenylphosphine)copper involves non-covalent interactions (such as groove binding mode) through the copper(I) center to affect DNA function, showing inhibitory activity against bacteria, fungi, and tumor cells. Chlorotris(triphenylphosphine)copper is promising for research of antibacterial, antitumor, and antioxidant agents.
    Chlorotris(triphenylphosphine)copper
  • HY-114227
    Hexidium iodide
    98.88%
    Hexidium iodide, a fluorescent nucleic binding acid stain (excitation/emission ~ 518/600 nm), permeants to mammalian cells and selectively stains almost all gram-positive bacteria. Hexidium iodide can bind to the DNA of all bacteria after permeabilization by EDTA.
    Hexidium iodide
  • HY-160841
    Biotin-16-dCTP
    Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation.
    Biotin-16-dCTP
  • HY-15625
    Hoechst 33258 analog 3
    99.82%
    Hoechst 33258 analog 3 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 analog 3
  • HY-D0828
    3,6-Dichlorotrimellitic acid
    99.31%
    3,6-Dichlorotrimellitic acid is the key precursor that is used for preparing a variety of dichlorinated fluoresceins and rhodamines such as TET and HEX.
    3,6-Dichlorotrimellitic acid
  • HY-15629
    HOE 32020
    98.07%
    HOE 32020 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    HOE 32020
  • HY-15622
    meta-iodoHoechst 33258
    98.66%
    meta-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    meta-iodoHoechst 33258
  • HY-15628
    Hoechst 33258 analog 5
    98.19%
    Hoechst 33258 analog 5 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 analog 5
  • HY-15624
    Hoechst 33258 analog 2
    98.41%
    Hoechst 33258 analog 2 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 analog 2